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<t>AAV2-retro</t> <t>preferentially</t> transduces photoreceptors and RPE 1 and 3 days after intravitreal injection. ( A , C ) Stitched 10× images of retinal cross-sections showing mGL expression at 1 dpi ( A ) and at 3 dpi ( C ). Blue indicates DAPI-labeled nuclei; green indicates mGL reporter expression. ( B , D ) Higher-magnification images of representative retinal regions showing mGL expression at 1 dpi ( B ) and at 3 dpi ( D ). ( B’ , D’ ) Merged images corresponding to panels B and D , illustrating mGL and DAPI expression across retinal layers. Retinal layers are indicated: RPE, ONL, OPL, INL, inner plexiform layer (IPL), and GCL. Scale bars : 200 µm ( A , C ); 50 µm ( B’ , D’ ).

Aav2 Retro, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 90 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/raav2-retro/rAAV2-retro+helper+(Plasmid+%2381070)/pmc13037736-28-7-19
Average 95 stars, based on 90 article reviews

aav2 retro - by Bioz Stars, 2026-09

95/100 stars

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1) Product Images from "AAV2-Retro–Mediated Gene Transfer Selectively Targets Outer Retinal Cells Following Intravitreal Injection"

Article Title: AAV2-Retro–Mediated Gene Transfer Selectively Targets Outer Retinal Cells Following Intravitreal Injection

Journal: Investigative Ophthalmology & Visual Science

doi: 10.1167/iovs.67.3.54

AAV2-retro preferentially transduces photoreceptors and RPE 1 and 3 days after intravitreal injection. ( A , C ) Stitched 10× images of retinal cross-sections showing mGL expression at 1 dpi ( A ) and at 3 dpi ( C ). Blue indicates DAPI-labeled nuclei; green indicates mGL reporter expression. ( B , D ) Higher-magnification images of representative retinal regions showing mGL expression at 1 dpi ( B ) and at 3 dpi ( D ). ( B’ , D’ ) Merged images corresponding to panels B and D , illustrating mGL and DAPI expression across retinal layers. Retinal layers are indicated: RPE, ONL, OPL, INL, inner plexiform layer (IPL), and GCL. Scale bars : 200 µm ( A , C ); 50 µm ( B’ , D’ ).

Figure Legend Snippet: AAV2-retro preferentially transduces photoreceptors and RPE 1 and 3 days after intravitreal injection. ( A , C ) Stitched 10× images of retinal cross-sections showing mGL expression at 1 dpi ( A ) and at 3 dpi ( C ). Blue indicates DAPI-labeled nuclei; green indicates mGL reporter expression. ( B , D ) Higher-magnification images of representative retinal regions showing mGL expression at 1 dpi ( B ) and at 3 dpi ( D ). ( B’ , D’ ) Merged images corresponding to panels B and D , illustrating mGL and DAPI expression across retinal layers. Retinal layers are indicated: RPE, ONL, OPL, INL, inner plexiform layer (IPL), and GCL. Scale bars : 200 µm ( A , C ); 50 µm ( B’ , D’ ).

Techniques Used: Injection, Expressing, Labeling

AAV2-retro maintains selective transduction in the outer layers 14 days after intravitreal injection. ( A ) Stitched 10× images of retinal cross-sections showing mGL expression at 14 dpi. ( B ) Higher-magnification view of a representative retinal region with mGL expression. ( B’ ) Merged image from B highlighting layer-specific distribution (RPE, ONL, OPL, INL, IPL, and GCL), mGL, and DAPI across the layers. ( C ) Retinal flatmount at 14 dpi after sequential injections, showing widespread mGL expression. ( C’ , C’’ ) Higher magnification images of the boxed areas in C . Scale bars : 200 µm ( A , C ); 50 µm ( B’ , C’ , C’’ ). ( D ) Quantification of mGL-positive cells within the regions of high transduction in the ONL (i.e., ROI). The percentage shown indicates the proportion of transduced cells among total DAPI-positive ONL cells. N = 3 animals. Scale bars : 200 µm ( A , C ); 50 µm ( C’ , C’’ ).

Figure Legend Snippet: AAV2-retro maintains selective transduction in the outer layers 14 days after intravitreal injection. ( A ) Stitched 10× images of retinal cross-sections showing mGL expression at 14 dpi. ( B ) Higher-magnification view of a representative retinal region with mGL expression. ( B’ ) Merged image from B highlighting layer-specific distribution (RPE, ONL, OPL, INL, IPL, and GCL), mGL, and DAPI across the layers. ( C ) Retinal flatmount at 14 dpi after sequential injections, showing widespread mGL expression. ( C’ , C’’ ) Higher magnification images of the boxed areas in C . Scale bars : 200 µm ( A , C ); 50 µm ( B’ , C’ , C’’ ). ( D ) Quantification of mGL-positive cells within the regions of high transduction in the ONL (i.e., ROI). The percentage shown indicates the proportion of transduced cells among total DAPI-positive ONL cells. N = 3 animals. Scale bars : 200 µm ( A , C ); 50 µm ( C’ , C’’ ).

Techniques Used: Transduction, Injection, Expressing

AAV2-retro leads to similar preferential transduction in female mice after intravitreal injection. ( A , C ) Stitched 10× images of retinal cross-sections showing mGL expression at 3 dpi ( A ) and at 14 dpi ( C ). Blue indicates DAPI-labeled nuclei; green indicates mGL reporter expression. ( B , D ) Higher magnification images of representative retinal regions showing mGL expression at 3 dpi ( B ) and at 14 dpi ( D ). ( B’ , D’ ) Merged images corresponding to panels B and D , illustrating mGL and DAPI expression across retinal layers. Retinal layers are indicated: RPE, ONL, OPL, INL, IPL, and GCL. Three female mice were examined per time point with similar results. Scale bars : 200 µm ( A , C ); 50 µm ( B’ , D’ ).

Figure Legend Snippet: AAV2-retro leads to similar preferential transduction in female mice after intravitreal injection. ( A , C ) Stitched 10× images of retinal cross-sections showing mGL expression at 3 dpi ( A ) and at 14 dpi ( C ). Blue indicates DAPI-labeled nuclei; green indicates mGL reporter expression. ( B , D ) Higher magnification images of representative retinal regions showing mGL expression at 3 dpi ( B ) and at 14 dpi ( D ). ( B’ , D’ ) Merged images corresponding to panels B and D , illustrating mGL and DAPI expression across retinal layers. Retinal layers are indicated: RPE, ONL, OPL, INL, IPL, and GCL. Three female mice were examined per time point with similar results. Scale bars : 200 µm ( A , C ); 50 µm ( B’ , D’ ).

Techniques Used: Transduction, Injection, Expressing, Labeling

AAV2-retro effectively delivers transgene to both rods and cones. ( A , B ) Representative retinal cross-sections showing mGL expression ( green ) at 3 dpi ( A ) and at 14 dpi ( B ) following intravitreal injection of AAV2-retro–CMV–mGL. Cone arrestin ( magenta ) was used to identify cone photoreceptors. Bottom panel in A shows higher magnification images highlighting the colocalization of mGL with cone arrestin immunoreactivity. Scale bars : 50 µm ( A , B ); 5 µm (higher magnification images).

Figure Legend Snippet: AAV2-retro effectively delivers transgene to both rods and cones. ( A , B ) Representative retinal cross-sections showing mGL expression ( green ) at 3 dpi ( A ) and at 14 dpi ( B ) following intravitreal injection of AAV2-retro–CMV–mGL. Cone arrestin ( magenta ) was used to identify cone photoreceptors. Bottom panel in A shows higher magnification images highlighting the colocalization of mGL with cone arrestin immunoreactivity. Scale bars : 50 µm ( A , B ); 5 µm (higher magnification images).

Techniques Used: Expressing, Injection

AAV2-retro transduces only a small number of amacrine cells and RGCs. ( A , B ) Retinal cross-sections show mGL expression ( green ) at 3 dpi ( A ) and at 14 dpi ( B ). AP-2α immunoreactivity is shown in magenta and RBPMS immunoreactivity in red . (A’ , B’ ) Higher magnification images showing cells that appear to be co-labeled with mGL and AP-2α. ( C ) Quantification shows that the percentage of mGL + cells that were positive for either RBPMS (in GCL and INL) or AP-2α at each time point. N = 3 per group. Error bar, ± SEM Two-way ANOVA with Tukey's multiple comparisons: * < 0.05, **** < 0.0001, ns = not significant. Scale bars : 50 µm ( A , B ); 5 µm ( A’ , B’ ).

Figure Legend Snippet: AAV2-retro transduces only a small number of amacrine cells and RGCs. ( A , B ) Retinal cross-sections show mGL expression ( green ) at 3 dpi ( A ) and at 14 dpi ( B ). AP-2α immunoreactivity is shown in magenta and RBPMS immunoreactivity in red . (A’ , B’ ) Higher magnification images showing cells that appear to be co-labeled with mGL and AP-2α. ( C ) Quantification shows that the percentage of mGL + cells that were positive for either RBPMS (in GCL and INL) or AP-2α at each time point. N = 3 per group. Error bar, ± SEM Two-way ANOVA with Tukey's multiple comparisons: * < 0.05, **** < 0.0001, ns = not significant. Scale bars : 50 µm ( A , B ); 5 µm ( A’ , B’ ).

Techniques Used: Expressing, Labeling

Produced:

Article Title: AAV2-Retro-Mediated Gene Transfer Selectively Targets Outer Retinal Cells Following Intravitreal Injection
Article Snippet: .. AAVs were produced with two different capsids the AAV2-retro (rAAV2-retro helper was a gift from Alla Karpova & David Schaffer (Addgene plasmid # 81070; RRID:Addgene_81070) and MNM008. ..

Article Title: AAV2-Retro-Mediated Gene Transfer Selectively Targets Outer Retinal Cells Following Intravitreal Injection.
Article Snippet: .. AAVs were produced with two different capsids: AAV2-retro (rAAV2-retro helper was a gift from Alla Karpova and David Schaffer; Addgene plasmid #81070; RRID:Addgene_81070) and MNM008.11 The MNM008 capsid was constructed on the AAV2-retro backbone plasmid by substituting the sequence between N587 and R602 of the AAV2-retro VP1 capsid gene with the amino acid sequence SFTSPLHKNENTVS. ..

Article Title: Retrograde viral delivery of hyper-IL-6 activates Stat3 in corticospinal tract neurons but causes severe tremors and weight loss in adult mice
Article Snippet: The resulting sequence was codon-optimized using a Genscript algorithm for mouse expression and synthesized by Genscript with flanking KpnI and EcoRI restriction sites. .. The resulting construct was inserted into an AAV construct with a CAG promoter, using (Addgene# 191093). rAAV2-retro-Hyper-IL-6 was then produced at the University of Miami viral core at a titer of 2.1x10 14 . .. Prior to injection all viruses were diluted in Phosphate Buffered Saline (PBS: Sigma P4474, 154mM sodium chloride, 1.058mM potassium phosphate monobasic, 2.97mM sodium phosphate dibasic dihydrate, pH 7.4) with 5% D-Sorbitol (Sigma S1876) and 0.001% Poloxamer 188 Surfactant (ThermoFisher 24040032).

Article Title: Descending locus coeruleus noradrenergic signaling to spinal astrocyte subset is required for stress-induced mechanical pain hypersensitivity
Article Snippet: .. The SaCas9 cassette was transferred into pAAV-CMV-FLEx-WPRE plasmid to generate pAAV-CMV-FLEx[SaCas9]-WPRE. rAAV vectors were produced from human embryonic kidney 293T (HEK293T) cells (#632273, Takara Bio, Shiga, Japan; mycoplasma testing was not independently performed in this study; however, no signs of contamination were observed) with triple transfection [pZac or pAAV, cis plasmid; pAAV2/5 (University of Pennsylvania Gene Therapy Program Vector Core), pAAV2/9 (University of Pennsylvania Gene Therapy Program Vector Core) or pAAV2/retro (#81070; Addgene), trans plasmid; pAd DeltaF6, adenoviral helper plasmid (University of Pennsylvania Gene Therapy Program Vector Core)] and purified by two cesium chloride density gradient purification steps. .. The vector was dialyzed against phosphate-buffered saline (PBS) containing 0.001% (vol/vol) Pluronic-F68 (#24040032; Thermo Fisher Scientific) using Vivaspin Turbo 15 100,000 MWCO (#VS15T41; Sartorius).

Article Title: AAV2-Retro–Mediated Gene Transfer Selectively Targets Outer Retinal Cells Following Intravitreal Injection
Article Snippet: .. AAVs were produced with two different capsids: AAV2-retro (rAAV2-retro helper was a gift from Alla Karpova and David Schaffer; Addgene plasmid #81070; RRID:Addgene_81070) and MNM008. ..

Article Title: Retrograde viral delivery of hyper-IL-6 activates Stat3 in corticospinal tract neurons but causes severe tremors and weight loss in adult mice
Article Snippet: .. rAAV2-retro-CAG-H2B-mGreenLantern (Addgene#177332) was produced at the University of Miami viral core facility at the Miami Project to Cure Paralysis, titer = 1.4x10 13 particles/ml. rAAV2-retro-tdTomato (Addgene #59462) were produced at the University of North Carolina Viral Vector Core at 3x10 12 particles/ml. ..

Article Title: Descending locus coeruleus noradrenergic signaling to spinal astrocyte subset is required for stress-induced mechanical pain hypersensitivity
Article Snippet: .. The SaCas9 cassette was transferred into pAAV- CMV- FLEx- WPRE plasmid to generate pAAV- CMV- FLEx[SaCas9]-WPRE. rAAV vectors were produced from human embryonic kidney 293T (HEK293T) cells (#632273, Takara Bio, Shiga, Japan; mycoplasma testing was not independently performed in this study; however, no signs of contamination were observed) with triple transfection [pZac or pAAV, cis plasmid; pAAV2/5 (University of Pennsylvania Gene Therapy Program Vector Core), pAAV2/9 (University of Pennsylvania Gene Therapy Program Vector Core) or pAAV2/retro (#81070; Addgene), trans plasmid; pAd DeltaF6, adenoviral helper plasmid (University of Pennsylvania Gene Therapy Program Vector Core)] and purified by two cesium chloride density gradient purification steps. .. The vector was dialyzed against phosphatebuffered saline (PBS) containing 0.001% (vol/vol) Pluronic- F68 (#24040032; Thermo Fisher Scientific) using Vivaspin Turbo 15 100,000 MWCO (#VS15T41; Sartorius).

Plasmid Preparation:

Article Title: Disruption of the Brain-Spleen Axis Impairs Monocyte-Microglia Communication and Accelerates Disease Progression in a Model of Amyloidosis
Article Snippet: .. AAV generation: To produce rAAV2-retro, a triple co-transfection procedure was used to introduce a rAAV vector plasmids together with rAAV2-retro, helper plasmid carrying AAV rep and cap genes (rAAV2-retro helper was a gift from Alla Karpova and David Schaffer (Addgene plasmid #81070; http://n2t.net/addgene:81070 ; RRID:Addgene_81070)) and pXX6-80, Ad helper plasmid, at a 1:1:1 molar ratio( ). .. Briefly, HEK293T cells were transfected using poly- ethylenimine (PEI) (linear; molecular weight [MW], 25,000) (Poly- sciences, Inc., Warrington, PA), and medium was replaced at 18 h post- transfection.

Article Title: Descending locus coeruleus noradrenergic signaling to spinal astrocyte subset is required for stress-induced mechanical pain hypersensitivity
Article Snippet: .. The SaCas9 cassette was transferred into pAAV- CMV- FLEx- WPRE plasmid to generate pAAV- CMV- FLEx[SaCas9]-WPRE. rAAV vectors were produced from human embryonic kidney 293T (HEK293T) cells (#632273, Takara Bio, Shiga, Japan; mycoplasma testing was not independently performed in this study; however, no signs of contamination were observed) with triple transfection [pZac or pAAV, cis plasmid; pAAV2/5 (University of Pennsylvania Gene Therapy Program Vector Core), pAAV2/9 (University of Pennsylvania Gene Therapy Program Vector Core) or pAAV2/retro (#81070; Addgene), trans plasmid; pAd DeltaF6, adenoviral helper plasmid (University of Pennsylvania Gene Therapy Program Vector Core)] and purified by two cesium chloride density gradient purification steps. .. The vector was dialyzed against phosphatebuffered saline (PBS) containing 0.001% (vol/vol) Pluronic- F68 (#24040032; Thermo Fisher Scientific) using Vivaspin Turbo 15 100,000 MWCO (#VS15T41; Sartorius).

Construct:

Sequencing:

Bioprocessing:

Transfection:

Article Title: Descending locus coeruleus noradrenergic signaling to spinal astrocyte subset is required for stress-induced mechanical pain hypersensitivity
Article Snippet: .. The SaCas9 cassette was transferred into pAAV- CMV- FLEx- WPRE plasmid to generate pAAV- CMV- FLEx[SaCas9]-WPRE. rAAV vectors were produced from human embryonic kidney 293T (HEK293T) cells (#632273, Takara Bio, Shiga, Japan; mycoplasma testing was not independently performed in this study; however, no signs of contamination were observed) with triple transfection [pZac or pAAV, cis plasmid; pAAV2/5 (University of Pennsylvania Gene Therapy Program Vector Core), pAAV2/9 (University of Pennsylvania Gene Therapy Program Vector Core) or pAAV2/retro (#81070; Addgene), trans plasmid; pAd DeltaF6, adenoviral helper plasmid (University of Pennsylvania Gene Therapy Program Vector Core)] and purified by two cesium chloride density gradient purification steps. .. The vector was dialyzed against phosphatebuffered saline (PBS) containing 0.001% (vol/vol) Pluronic- F68 (#24040032; Thermo Fisher Scientific) using Vivaspin Turbo 15 100,000 MWCO (#VS15T41; Sartorius).

Purification:

Article Title: Descending locus coeruleus noradrenergic signaling to spinal astrocyte subset is required for stress-induced mechanical pain hypersensitivity
Article Snippet: .. The SaCas9 cassette was transferred into pAAV- CMV- FLEx- WPRE plasmid to generate pAAV- CMV- FLEx[SaCas9]-WPRE. rAAV vectors were produced from human embryonic kidney 293T (HEK293T) cells (#632273, Takara Bio, Shiga, Japan; mycoplasma testing was not independently performed in this study; however, no signs of contamination were observed) with triple transfection [pZac or pAAV, cis plasmid; pAAV2/5 (University of Pennsylvania Gene Therapy Program Vector Core), pAAV2/9 (University of Pennsylvania Gene Therapy Program Vector Core) or pAAV2/retro (#81070; Addgene), trans plasmid; pAd DeltaF6, adenoviral helper plasmid (University of Pennsylvania Gene Therapy Program Vector Core)] and purified by two cesium chloride density gradient purification steps. .. The vector was dialyzed against phosphatebuffered saline (PBS) containing 0.001% (vol/vol) Pluronic- F68 (#24040032; Thermo Fisher Scientific) using Vivaspin Turbo 15 100,000 MWCO (#VS15T41; Sartorius).

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